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Automacs Cell Separator Magnetic Beads

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  • (PDF) On chip cell   separator using magnetic bead …

    (PDF) On chip cell separator using magnetic bead …

    On chip cell separator using magnetic bead-based enrichment and depletion of various surface markers ... step using a panel of eight antibodies in combination with MACS magnetic beads and an ...

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  • Magnetic separation of   organelles using magnetic …

    Magnetic separation of organelles using magnetic …

    2019-6-25 · The cells lipofected with hybrid nanoparticles were detached from the culture dish using a cell lifter at the appropriate time and homogenized. The resultant cell lysates were subjected to magnetic separation using an autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany).

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  • Influence of NK cell magnetic bead   isolation methods …

    Influence of NK cell magnetic bead isolation methods …

    Influence of NK cell magnetic bead isolation methods on phenotype and function of murine NK cells ... All separation protocols described in this paper were performed by the use of magnetic beads on the autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). After the different cell isolation procedures, we harvested the NK cell ...

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  • Isolate Specific Cell Populations   with Magnetic …

    Isolate Specific Cell Populations with Magnetic …

    But when quick, inexpensive cell purification is the goal, many turn instead to magnetic beads. Magnetic beads can divide and capture a cell type of interest within a half-hour. Aided by antibodies to bind the desired cells within a mixed population, the beads stick to …

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  • Fundamentals and Application of Magnetic   Particles …

    Fundamentals and Application of Magnetic Particles …

    Magnetic Beads for Cell Separation Applications . The first parameter that will be examined in the context of optimizing magnetic cell separation platforms is the magnetic beads themselves, with factors such as materials selection, synthesis methods, and particle size to be considered.

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  • autoMACS® Pro Separator -   file.yizimg.com

    autoMACS® Pro Separator - file.yizimg.com

    2015-11-20 · Performing cell separations has never been easier with the autoMACS® Pro Separator. This innovative instrument eliminates the need for manual sample handling steps, saving time while providing reliability and reproducibility in cell sorting. The autoMACS Pro Separator has been specifically designed for automated handling of multiple samples.

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  • Re-using MACS magnetic separation   beads? - …

    Re-using MACS magnetic separation beads? - …

    Re-using MACS magnetic separation beads? Has anyone had experience or heard of re-using MACs magnetic beads for cell isolation? Our lab isolates CD4+CD25+/- T cell frequently, and we go through ...

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  • Miltenyi Biotec AutoMacs   Pro Cell Sorter

    Miltenyi Biotec AutoMacs Pro Cell Sorter

    2019-11-12 · The built in LCD screen of this cell separator provides straightforward operation, and continuously monitors the processing of the sample. The Miltenyi Biotec autoMACS Pro cell sorter also uses an automated sensor controlled procedures guaranteeing the absolute safety of your samples.

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  • Cell death induced by the   application of alternating ...

    Cell death induced by the application of alternating ...

    Cell death induced by the application of alternating magnetic fields to ... with magnetic beads (CD14 Microbeads, Miltenyi) by positive immunoselection using the autoMACS Separator (Miltenyi). CD14+ cells (106 cells ml−1) were cultured in RPMI 1640 (Sigma) with 10% FBS, 1% glutamine, 1% antibiotics, IL-

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  • Method for Discovery of Peptide Reagents   Using a ...

    Method for Discovery of Peptide Reagents Using a ...

    2017-12-4 · widespread use is limited, in part due to poor performance achieved using manual Magnetic-Activated Cell Sorting (MACS) methods, and an absence of widely-available, low cost, high-performance sorting alternatives. Here, we have developed a methodology for bacterial cell sorting using the semi-automated autoMACS ® Pro Separator for

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  • macrophage(CD11b   beads)巨噬细胞磁珠分选说明_文档下载

    macrophage(CD11b beads)巨噬细胞磁珠分选说明_文档下载

    2015-11-5 · Then the cell suspension is loaded onto a MACS® Column which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD11b+ cells are retained on the column. The unlabeled cells run through and this cell fraction is depleted of

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  • Magnetic beads separator   rack for Ampure beads …

    Magnetic beads separator rack for Ampure beads …

    Just insert your PCR tube filled with magnetic beads suspension into a well of the separator, and wait for 1-3 minutes. ... Miltenyi Biotec AutoMACS 003 Automatic Laboratory Magnetic Cell Separator. AU $658.89 ... Details about Magnetic beads separator rack for Ampure beads made in 96 wells plate format.

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  • macrophage(CD11b   beads)巨噬细胞磁珠分选说明_文库下载

    macrophage(CD11b beads)巨噬细胞磁珠分选说明_文库下载

    2015-11-5 · Then the cell suspension is loaded onto a MACS® Column which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD11b+ cells are retained on the column. The unlabeled cells run through and this cell fraction is depleted of

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  • Immunomagnetic   isolation of myeloma cells by …

    Immunomagnetic isolation of myeloma cells by …

    The standard method used for cell separation is Magnetic Antibody Cell Separation, MACS. In our laboratories, we use autoMACS separator (Miltenyi Biotec, Bergisch Gladbach, Germany) designed for positive selection as well as depletion of magnetically labeled cells.

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  • Analysis   of Tumor-infiltrating Lymphocytes Following CD45 ...

    Analysis of Tumor-infiltrating Lymphocytes Following CD45 ...

    A rough total cell count was performed using a haemocytometer to determine the amount of MACS beads required. CD45 + cell selection by magnetic cell sorting with the AutoMACS Tumor cells were transferred to 50 ml Falcon tubes and resuspended in 90 µl 7

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  • DS_CD11c MicroBeads_m_百度文库

    DS_CD11c MicroBeads_m_百度文库

    2016-7-24 · Collect total effluent; this is the unlabeled cell fraction. Depletion with D Columns For instructions on column assembly and separation refer to the D Column data sheet. Magnetic separation with the autoMACS? Pro Separator ?Refer to the respective user

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  • Anti-FITC MicroBeads

    Anti-FITC MicroBeads

    2014-1-18 · Magnetic cell sorting Anti-FITC MicroBeads 140-000-053.06 Order No. 130-048-701. ... Prepare and prime autoMACS Separator. 2. Place tube containing magnetically labeled cells in autoMACS. Choose a separation program according to the recommendations in the autoMACS …

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  • Isolating Myeloid Derived   Suppressor Cells(MDSC) …

    Isolating Myeloid Derived Suppressor Cells(MDSC) …

    This is a rapid and comprehensive method of immunophenotyping Myeloid Derived Suppressor Cells (MDSC) and enriching Gr-1 + leukocytes from mouse spleens. This method uses flow cytometry and AutoMACS Cell Sorting to enrich for viable Gr-1 + leukocytes prior to FACS sorting of MDSC for use in vivo and in vitro assays. Cite this Article

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  • CD4 (L3T4) MicroBeads   mouse

    CD4 (L3T4) MicroBeads mouse

    2019-1-28 · MicroBeads. Then, the cell suspension is loaded onto a MACS® column which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD4+ cells are retained on the column. The unlabeled cells run through and this cell fraction is depleted of CD4+ cells. After removal of the column from the magnetic field,

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  • Anti-Biotin MicroBeads

    Anti-Biotin MicroBeads

    2014-1-18 · Anti-Biotin MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled material is retained within the column. The unlabeled material runs through; this cell fraction is thus depleted of magnetically labeled material. After removing the

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  • Ethylene-Diamine-Tetra-Acetate (EDTA)   mimics the …

    Ethylene-Diamine-Tetra-Acetate (EDTA) mimics the …

    Cell separation was performed according to the manufacturer's instructions. For incubation with beads, we used MACS buffer (Bie & Berntsen, Denmark) whereas the AutoMACS Running Buffer (Miltenyi Biotec) was used for cell separations on the AutoMACS Cell Separator. CD4+ T cell purity and CD4+CD25+ T cell purity were above 97% and 85%, respectively.

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  • Miltenyi   Biotec - Wikipedia

    Miltenyi Biotec - Wikipedia

    2019-11-15 · Miltenyi Biotec is a global biotechnology company headquartered near Cologne in Bergisch Gladbach, Germany. The company is a provider of products and services that support scientists, clinical researchers, and physicians across basic research, translational research, and clinical applications. The company offers solutions[buzzword] covering ...

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  • CD45 MicroBeads mouse

    CD45 MicroBeads mouse

    2015-11-19 · cell labeling. Volumes for magnetic labeling given below are for up to 107 total cells. When working with fewer than 107 cells, use the same volumes as indicated. When working with higher cell numbers, scale up all ... use the autoMACS Separator. 1. Prepare and prime autoMACS Separator. page 2/3 140-000-074.05 Order No. 130-052-301.

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  • CELL SEPARATION-   Miltenyi

    CELL SEPARATION- Miltenyi

    2015-2-4 · autoMACS® Columns 130‐021‐101 5×2 columns 0 Columns for the autoMACS Pro Separator and the autoMACS Separator MultiMACS Cell24 Separator MultiMACS Cell24 Separator Plus 130‐098‐637 14.900,00 0 Instrument for automated magnetic cell separation from up …

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